应用案例
单抗分离重现性实验
2023-02-08
仪器推荐 |
SDA系列 SCG-P系列 |
|
设备/材料/参数 |
描述 |
备注 |
层析柱 |
16*300mm |
柱高:15±2cm;As:0.7~1.8;HETP:≥1000N/m |
保留时间 |
6min |
N/A |
DBC判定标准 |
DBC10% |
N/A |
样品 |
细胞澄清液 |
N/A |
溶液 |
参数 |
备注 |
亲和层析平衡液 |
20mM PB+0.1M NaCl |
pH:6.90-7.40conc.:10.00-14.00mS/cm |
亲和层析冲洗液I |
0.1M柠檬酸钠+0.5M NaCl |
pH:5.80-6.20conc.:55.0-65.0mS/cm |
亲和层析洗脱液 |
10mM NaAc-HAc |
pH:3.20-3.40conc.:0.20-0.30mS/cm |
亲和层析消毒液 |
0.1M NaOH |
N/A |
亲和层析保存液 |
N/A |
pH:5.00-5.40conc.:N/A |
循环制备 HCP、ProA等质量数据
序号 |
HCP |
ProA |
CE |
序号 |
HCP |
ProA |
CE |
ppm |
ppm |
% |
ppm |
ppm |
% |
||
1 |
154.44 |
7.56 |
97.97 |
63 |
122.38 |
4.85 |
97.65 |
2 |
105.21 |
7.17 |
97.87 |
81 |
171.84 |
7.81 |
97.62 |
3 |
137.17 |
5.96 |
97.81 |
99 |
94.72 |
5.46 |
97.72 |
9 |
85.19 |
6.92 |
97.80 |
117 |
105.51 |
9.28 |
97.86 |
18 |
87.35 |
5.32 |
97.84 |
126 |
113.22 |
5.67 |
97.74 |
27 |
154.44 |
11.14 |
97.52 |
135 |
107.74 |
5.06 |
97.68 |
45 |
167.49 |
7.98 |
97.68 |
144 |
172.63 |
5.67 |
97.70 |